Galli-maynini reaction

Reaction Galli-Maynini — method of diagnosis of early pregnancy. Carried out on the lake with male frogs. Reaction Galli-Maynini based on the fact that HCG and horionalny hormones in the urine of pregnant women, stimulate spermatogenesis in male frogs.
Technique. 3-4 ml of morning urine sample woman who is supposed pregnancy, injected frog in length from 5 to 7.5 cm in the lymph sac (located on the back). Puncture needle is almost parallel to the back of the frog, not to damage the lungs. 2-3 hours take the contents of the cloaca frogs examined under a microscope.
With the positive response observed turbidity drops by release of large amounts of sperm. Winter frogs kept in a warm place. Reaction Galli-Maynini faster than Ashgeyma — Tsondeka reaction (see), has a greater percentage of correct responses (95-96%), technically simpler. Galli-reaction Maynini can perform paramedic. The woman should be advised that delivers urine to the assay, restrict fluid intake to prevent the eve of large breeding hormones in urine.

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